The experiment was conducted at the Department of Physiology and Pharmacology, Hajee Mohammad Danesh Science and Technology University, Dinajpur. A total of 40 quails are randomly selected and divided into 4 groups (T0, T1, T2 and T3) for assessing the effect of ipil ipil and bean leaves supplementation on egg production, egg quality and growth performance of quail. Group T0; considered as control, fed only with commercial layer ration, group T1; supplemented with formulation of 2g grinded ipil ipil leaves per kg feed, group T2; supplemented with formulation of 2 g grinded bean leaves per kg feed and group T3; supplemented with formulation of 2 g grinded ipil ipil leaves with 2g grinded bean leaves per kg feed. All the quail of control and treated groups were closely observed for 4 weeks and following parameters were studied.
At 42 days of age, Japanese layer quails were collected from A. R. Enterprise (quail hatchery and farm), Bogra. Body weights of assigned quails were taken with digital weight balance and the data were recorded. The finally selected 40 quails were housed under normal husbandry condition and reared quail in quail cage. All the birds were fed with commercial crumbled and mesh feed at the rate of 30g per bird per day and fresh water ad-libitum. Measurement of body weight was performed with the help of digital balance.
Ipil ipil and bean leaves were collected from the Brac Nursery, Dinajpur. The ipil ipil and bean leaves were dried and grinded. The grinded leaves were added with commercial quail ration and served to recommended groups.
Egg production was recorded for each quail at the same time each day during laying period. The incidence of broken eggs and soft-shelled eggs were identified and recorded. The number of eggs laid on successive days by a particular quail determined the length of each sequence and the number of pauses in each quail’s oviposition determined the number of sequences. For each quail, the length of laying sequence was determined on the day the last egg of the quail was laid.
Egg qualities wear measured from those eggs laid by quails of different treatment group. Measured egg qualities were egg weight, shell dry weight, fresh albumin weight, fresh yolk weight, egg shell thickness, height of the thick albumin, height of the yolk, width of the yolk, width of the egg, and diameter of the egg albumin. For quality determination egg weight was recorded by an electric weighing balance. The length of egg was measured by a slide calipers. The width was also estimated by slide calipers. The eggs were then carefully broken down on a glass plate (40 × 20 cm) to determine the internal egg qualities.
The method outlined by Chowdhury (1988) was followed for partition in different egg components. At first, egg was broken on glass plate. Then the yolk was separated carefully from albumin with the help of a spatula and transferred to a previously weighed petridish by a spatula and weighed. Precautions wear taken at all stages to avoid rupture of yolk. The shell of the broken eggs wear rinsed and washed thoroughly in tap water keeping the membranes intake. The washed shells with membrane were immersed in a beaker of water for removal of the shell membranes. The shell and shell membranes were oven dried separately at 105°C over night keeping them in a glass petridish. On the following day, oven dried shell and shell membranes were taken. Finally the following calculations were made for different components suggested by Chowdhury (1988).
After removing of shell membrane, shell thickness (mm) was measured by screw gauge.
Data were analyzed by analysis of variance using Completely Randomized Design with factorial arrangement of time and treatments (Steel and Torrie, 1986). All analyses wear performed by MSTATC and SPSS program.
This study investigated the effect of ipil ipil and bean leaves supplementation on growth performance, egg production and egg quality of quail.
Egg production of different groups of quail were recorded from 6 weeks to 10 weeks quail treated with ipil ipil, bean leaves and combined ipil ipil plus bean leaves. The average egg production of different groups of quail were recorded. Quails treated with ipil ipil leaves showed average egg production 21.8 ± 0.32 within 06-10 weeks, bean leaves treated groups showed average egg production 22.5 ± 0.50 and combined treatment supplementation showed average egg production 23.3 ± .47 within 6 weeks to 10 weeks. (Table 1). Control group showed average egg production 17.5 ± 0.34. Result showed highest egg production in combined treatment group (23.3 ± 0.47) and lowest in control group (17.5 ± 0.34). Moreover, ipil ipil and bean leaves supplementation increase egg production. Our study express the same results.
|Treatment Groups||Egg production (06-10) weeks||Percentage (%)|
|T0 (control)||17.50c ± 0.34||45.71%|
|T1 (ipil ipil)||21.80b ± 0.33||55.47%|
|T2 (bean leaves)||22.50ab ± 0.50||57.38%|
|T3 (ipil ipil + bean)||23.30a ± 0.47||59.28%|
Note: Values followed by different superscripts in the same column a restatistically significant (p < 0.05).
Body weight of different groups of quails were recorded from 06 to 10 weeks quail treated with ipil ipil leaves, bean leaves and combined ipil ipil plus bean leaves. The average body weight of different group of quails were recorded. Quails treated with ipil ipil leaves showed average Body weight gain 123.5a ± 3.30 to 149.2a ± 5.14 gm within 06-10 weeks, bean leaves treated groups showed body weight gain 123.8a ± 3.42 to 149.0a ± 6.49 gm and combined treatment supplementation showed body weight 126.8a ± 2.78 to 155.1a ± 7.15 gm within 06-10 weeks (Table 2). Control group showed average body weight gain 128.5a ± 4.00 to 151.9a ± 5.48 gm. Result showed that the body weight of different groups more or less same with the control group. Results are contrary to that observed by Saima., et al. (2014) A 28 days’ trial was conducted to evaluate efficacy of microbial phytase in diets for Japanese quails. For this purpose, 900 experimental birds were divided into six groups, each group containing 150 chicks and further sub-divided into10 replicates. Diet A (positive control) was formulated according to NRC (1994) requirements set for the Japanese quail (CP 24% and ME 2900 Kcal/Kg). Diet B differed from diet A in Ca (Calcium) and P (Phosphorus) i.e. 0.15% Ca and 0.20% P less to Diet A, respectively. Four different levels of phytase enzyme (250, 500, 750, 1000 FTU/kg of feed) were added to diet B to formulate diets C, D, E and F treatments respectively. Results revealed that body weight gain, feed consumption, FCR, keel/shank length, dressing percentage of birds in groups consuming 750 and 1000 FTU/kg phytase were significantly higher (P < 0.05) than those of B, C and D. The growth performance of group E and F was comparable with those of group A (+ ve control). Maximum leg weakness, swollen joints and crippled legs were observed in group B (39.30%) followed by C, D (21.33% and 16.0%).
|Group||Body weight (gm.)|
|06 weeks (starting of the experiment)||End of 10 weeks|
|T0||128.5a ± 4.00||151.9a ± 5.48|
|T1||123.5a ± 3.30||149.2a ± 5.14|
|T2||123.8a ± 3.42||149.0a ± 6.49|
|T3||126.8a ± 2.78||155.1a ± 7.15|
N.B: Values followed by same superscripts in the same column are not statistically significant (p < 0.05).
(Table 3) demonstrate that there exist a significant (P < 0.05) difference among the mean values like Length of the egg (mm), height of the thick albumin (mm), diameter of the albumin (mm), shell thickness (mm) corresponding to the different level of ipil ipil and bean leaves treatment. But no significant (P > 0.05) difference among the mean values like weight of the egg (gm), width of the egg (mm), height of the yolk (mm), width of the yolk (mm), shell dry wt (gm) corresponding to the different level of ipil ipil and bean leaves treatment. These results indicate that treated with ipil ipil and bean leaves had no adverse effect on external and internal qualities of eggs.
|Parameter||Group T0 (Mean ± SEM)||Group T1 (Mean ± SEM)||Group T2 (Mean ± SEM)||Group T3 (Mean ± SEM)|
|Weight of the Egg (g)||09.70b ± 0.633||10.20b ± 0.512||09.80b ± 0.663||10.50a ± 0.341|
|Width of the Egg (mm)||25.67a ± 0.065||25.54a ± 0.060||25.14b ± 0.063||24.74c ± 0.182|
|Height of Thick Albumin (mm)||3.14b ± 0.073||3.39a ± 0.048||3.60a ± 0.097||3.54a ± 0.061|
|Length of Egg (mm)||32.66a ± 0.148||31.21b ± 0.301||30.98b ± 0.230||32.06a ± 0.249|
|45.59a ± 0.116||42.79c ± 0.257||43.76b ± 0.293||41.34d ± 0.242|
|Height of Yolk (mm)||6.61b ± 0.111||6.84ab ± 0.107||6.12c ± 0.086.||6.92a ± 0.103|
|Egg shell thickness (mm)||0.17b ±0.008||0.16c ± 0.009||0.12d ± 0.004||0.19a ± 0.007|
|Width of the yolk (mm)||29.52b ± 0.127||29.63b ± 0.109||28.15c ± 0.384||30.60a ± 0.285|
|Shell dry weight (g)||3.19ab ± 0.058||3.05b ± 0.050||3.13ab ±0.105||3.38a ± 0.089|
Note: Values followed by same superscripts in the same column are not statistically significant (p > 0.05), different superscripts indicate that difference is significant (P < 0.05).
* = Significance a t the 0.05% level
NS = Non significance
None to declare.
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