2Haematology and blood Transfusion Department Federal Medical Centre, Yenagoa Bayelsa State Nigeria
3Department of Gynecology and Obstetrics, Federal Medical Centre Yenagoa, Bayelsa state, Nigeria
4Department of Medical Laboratory Science, Federal University Otuoke, Bayelsa state, Nigeria
5Department of Medical Laboratory Science, Abia State University Uturu, Abia state, Nigeria
6Department of Medical Services, Federal University Otuoke, Bayelsa State, Nigeria
7Department of Biological Sciences, Faculty of Science, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria
8Department of Medical Microbiology and Parasitology, College of Health Sciences, Niger Delta University, Wilberforce Island, Bayelsa state, Nigeria
Subjects of this study were students of Madonna University, Elele, Nigeria. Typically, Rivers state is one of the states in the Niger Delta region of Nigeria. The Rivers state shares boundary with neighboring Niger Delta states including Bayelsa in the Western and southern region, Abia in the East. The climatic condition of the area is similar to other Niger Delta region previously described by Ben-Eledo., et al. (2017a,b); Seiyaboh., et al. (2017a-c); Seiyaboh and Izah (2017a,b); Ogamba., et al. (2015a,b,c; 2017a,b). Farming of food crops such oil palm and cassava is a major economic activity of the resident of the area.
Inclusion Criteria: Subjects for this research were students of Madonna University, Elele. Patients that tested positive for H. pylori using one step anti-HP rapid screen test kit (Lot. Number: 20161115) were selected for the study. A total of 17 female Helicobacter pylori infected patients and 15 male Helicobacter pylori infected patients within the age of 18–32 years participated in this study. Another 21 and 20 age-matched control subjects for males and females respectively who were negative for H. pylori were selected.
Blood samples was collected following standard venipuncture technique previously described by Eledo., et al. (2017a, b). Approximately 5 ml of blood was collected from each subject from the anticubital or dorsal vein. About 2.25 ml of blood meant for prothrombin time and activated partial thromboplastin time was dispensed into plastic tube containing 0.25 ml of trisodium citrate, while the remaining 2.75 mls of blood mean for platelets analysis was dispensed into dipotassium EDTA bottles.
The methodology previously applied by Eledo., et al. (2017a,b) was adopted for the determination of prothrombin time. The kit used was from Agappe Diagnostics Switzerland. The PT REAGENT was pre-warmed to 37ºC for 10 minutes. Then 0.1 ml of plasma was dispensed into test cuvette at 37ºC and incubated for 3 minutes. About 0.2 ml of pre-warmed PT REAGENT was forcibly added into the test cuvette. The timer was started simultaneously and the time for the first clot to appear was recorded in seconds.
The methodology previously applied by Eledo., et al. (2017a,b) was adopted for the determination of activated partial prothrombin time. Reagent 1 (Cacl2) and reagent 2 (APTT REAGENT) were pre-warmed at 37ºC. Then 0.1 ml of the plasma was dispensed into test cuvettes at 37ºC. Then after, 0.1 ml of the pre-warmed reagent was added into the test cuvettes, which was well mixed and incubated at 37ºC for approximately 3 minutes. In addition, 0.1 ml of pre-warmed reagent 1 was added into the test cuvettes. The timer was started simultaneously and the time for the first clot to appear was taken.
Platelets count was determined based on the Cronkite’s ammonium oxalate method previously applied by Eledo., et al. (2017a,b). Ammonium oxalate was used as the diluent. A 1:20 dilution of blood was made by adding 0.1 ml of blood to 1.9 ml of diluents. The suspension was thoroughly mixed in an improved Neubauer counting chamber. The mixed settled suspension was viewed under x40 objective and the platelets counted. The number of platelets per liter was calculated from the formula:
SPSS software version 20 was used to carry out statistical analysis. Data were presented and mean ± standard error. The haemostatic values obtained among H. pylori patients were compared with the control subjects using “t” test at P < 0.05.
|Parameters||Mean ± standard error||t-value||P-value|
|Subjects (n = 17)||Control (n = 20)|
|Prothrombin time (PT), secs||15.50 ± 0.18||11.75 ± 0.19||14.232||0.000|
|Activated partial thromboplastin time (APTT), sec||34.53 ± 0.42||28.50 ± 0.65||7.440||0.000|
|Platelets counts (PLT)(x109/L)||186.24 ± 7.14||271.55 ± 11.71||-5.958||0.000|
|Parameters||Mean ± standard error||t-value||P-value|
|Subjects (n = 15)||Control (n = 21)|
|Prothrombin time (PT), secs||13.86 ± 0.27||11.24 ± 0.54||3.861||0.000|
|Activated partial thromboplastin time (APTT), sec||32.20 ± 0.84||29.05 ± 0.62||3.091||0.004|
|Platelets counts (PLT)(x109/L)||188.53 ± 10.72||274.81 ± 11.15||-5.385||0.000|
Permission was obtained from the ethics committees of the Medical Laboratory Science Department of Madonna University, Elele, Nigeria. Informed consent was obtained from the patients prior to sample collections.
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