Research Article
Volume 2 Issue 1 - 2018
Effect of Various Stress Relaxation Exercises on Electroencephalography, Sympathetic Skin Response and Type-2 Diabetes Mellitus
Dr. Tarun Saxena1*, Dr. Sanjay Patidar2, Dr. Azeema Ozefa Ali3, Manjari Saxena4, and Dr. Madhu Kabra5
1MD (Internal Medicine), Senior Consultant, Department of Internal Medicine,
2MD (Community Medicine & Biostatistics), honorary consultant
3B.H.M.S. Resident Doctor
4M. SC. PGDYHS Department Yoga and Physical education
5MD (Pathology), senior consultant, department of Pathology and Biochemistry Mittal Hospital and Research Centre, Ajmer, Rajasthan, India
*Corresponding Author: Tarun Saxena, MD (Internal Medicine), Senior Consultant, Department of Internal Medicine, Mittal Hospital and Research Centre, Ajmer, Rajasthan, India.
Received: September 01, 2018; Published: September 13, 2018
Abstract
Background/objectives: Type 2 diabetes is a multifactor disease. Various studies have linked stress and diabetes. Still prospective study assessing the effect of various stress relaxation methods on glycemic parameters, lipid profile, EEG and SSR is lacking. Therefore the study was planned to see the effects of various stress relaxation methods in management of diabetes.
Methods: This is a randomized double-blind, interventional, active control trial. Arbitrarily, a sample of 50 confirmed cases of type-2 diabetes mellitus was selected for the study. Cases were randomly divided into 2 groups; group 1 (n = 30 control). Group 2 interventional group (n = 20). Group 2 was again subdivided into two subgroups 2A (n = 10) who practiced yogic relaxation exercises like pranayama, asanas and kriyas, subgroup 2B (n = 10) who practiced Vipassana mental relaxation technique. Stress scaling (perceived stress scale/ PSS), mental health life style factors (i.e. working mental hours, sleep duration etc. were assessed. Examination included, Waist: Hip ratio (W: H), EEG (electroencephalography), SSR (sympathetic skin response), fasting and post prandial glucose (FBG/PPBG), Glycosylated hemoglobin (HbA1c) and lipid profile initial and after 12 weeks.
Results: Initially all groups were comparable, in W: H ratio, FBG/PPBG/HbA1c, lipid profile, EEG and SSR findings. (Desynchronized activity in EEG, increased basal sympathetic discharge, high FBG/PPBG/HbA1c, impaired lipid profile). After 12 weeks sub group 2 B who practiced Vipassana showed synchronization in EEG, reduction in basal sympathetic discharge, and significant improvement in FBG/PPBG/HbA1c and lipid profile.
Conclusion: Chronic mental exhaustion and chronic stress are desynchronized state of brain, associated with increased basal sympathetic discharge. Increase in sympathetic discharge produces insulin resistance, impaired insulin release and diabetes. Vipassana/mental rest reduces expenditure of neurotransmitters, reduces stress, synchronizes brain, reduces basal sympathetic discharge and helps in control of diabetes.
Keywords: Stress; Type- 2 diabetes mellitus; Electro-encephalography; Sympathetic skin response
Introduction
Type 2 diabetes mellitus is a disease characterized by chronic hyperglycemia and multisystem complications, which is an important cause of morbidity and mortality. Besides sedentary lifestyle/physical inactivity, genetic defect, obesity, diet, environmental factors, stress is an important risk factor in causation or initiation of the disease. (Park & Park 2017) [1]
According to Dorland’s medical dictionary, stress is the sum of biological reactions to any adverse stimulus, physical, mental or emotional, internal that tends to disturb an organism’s internal homeostasis. [2] It may be external (environmental, psychological, or social situations) or internal (illness).
During past few years many working people have documented association between stress and type- 2 diabetes mellitus. Saxena., et al. (2013) have found a clear association between chronic stress, mental exhaustion and diabetes. The study also has correlated findings with EEG (electroencephalography) and SSR (sympathetic skin response) [3]. Another study by Harris et al. has found that stress increases the rise of tye-2 diabetes onset in women [4]. Few more studies have linked stress and diabetes [5-11]. Demoski (1970) also reported that stress intensifies the known preexistent diabetes mellitus, brings the unrecognized diabetes to clinical recognition and may convert pre-diabetes to frank diabetes mellitus [12]. All these previous studies indicate the role of stress in diabetes. Still there is insufficient literature about prospective work assessing reduction in stress and mental exhaustion and its effect on glycemic parameters, lipid profile, EEG and SSR. Therefore the study was planned to assess the effect of various stress relaxation methods to reduce stress/mental exhaustion in management of diabetes [13-18].
Materials and Methods
Study type: This is a randomized, double-blind, interventional, parallel group, active control trial. The study was done in the department of Internal Medicine and the department of Yoga and Physical health in Mittal hospital and Research Centre, Pushkar road Ajmer, and Keshvam hospital Maharana pratap Nagar Ajmer, Rajasthan, India. Patients in these institutes come from all the parts of the city and nearby villages (encompassing an area of 20-25 kilometers) representing a population of twenty lakhs.
Case selection, study period and study methods: Arbitrarily, a sample of 50 confirmed cases of type-2 diabetes mellitus coming regularly to the hospital for the last two years were selected for the study. Informed written consent and approval of the institutional ethical committee was taken (Initial diagnosis based on glycosylated hemoglobin (HbA1c > 6.5%). Cases were randomly divided into 2 groups; group 1 (n = 30 control). Group 2 interventional group (n = 20) (group 2). Group 2 was again randomly subdivided into two subgroups; subgroup 2A (n = 10) who practiced various yogic relaxation exercises like pranayama, asanas and kriyas, subgroup 2B (n = 10) who practiced Vipassana mental relaxation technique. The age group of the selected cases was between 45-50 years, comprising both males and females. All groups and subgroups were age and sex matched. The patients were studied from January 2015 to December 2017. The symptoms, anthropometric measurements (W: H ratio-waist hip ratio) stress scaling, and EEG, SSR was recorded by specific technician in the hospital and checked by a consultant on that day. The following parameters were taken into consideration-.
Detailed history including family history
Anthropometric measurements (W: H ratio-waist hip ratio)
Vital parameters (resting pulse rate, blood pressure, respiratory rate)
Stress scaling: Various factors were analyzed through a questionnaire based mainly on PSS (Perceived stress scale) with rating 0,1,2,3 or 4 which indicates severity; 0= never, 1= almost never,2 = sometimes, 3 = fairly often, 4 = very often (total 17 items/points in stress with maximum score of 68; normal range 0-16, mild stress 17-20, moderate 21-30, severe > 30/68). Various points examined were – attached with anxiety, tension, flow/crowding of ideas, loss of control etc. [19]
Subjective assessment: History of various mental health life style factors (i.e. working mental hours, sleep duration etc. duration of presence of above habit [3]
EEG (Electroencephalography): initial and after 12 weeks EEG was done on a 16 channel system in a dark silent room. Basal rhythm was observed in eye closure after few minutes in a relaxed state. (RMS Recorder and Medicare system) [3]
SSR (sympathetic skin response) initial and after 12 weeks; SSR was done with standard protocol in supine, relaxed, semi darkened room with ambient temperature control at 22-24°C, in the upper limbs. Hand grip and cold presser was used as provocative methods. Skin potential changes during and between the tests were analyzed by a computer. Latency was measured from the onset of stimulus artifact to the beginning of response. Amplitude was recorded peak to peak. (RMS Recorder and Medicare system) [20,21]
Blood investigations: Dry Chemistry (vitros 250 by ortho) method was used.
Glycemic parameters
FBG- Fasting blood glucose (Glucose oxidase method) [22]
PPBG- Post prandial blood glucose (Glucose oxidase method) [22]
HbA1c-Glycosylated hemoglobin (Turbid metric immunoassay method) [23]
Lipid profile
Tgl- Triglyceride (Enzymatic method) [24]
HDL (High density lipoprotein)cholesterol (Non HDL precipitation method followed by enzymatic reaction method)
LDL (low density lipoprotein) & VLDL (very low density lipoprotein) Estimations- Calculated
Exclusion Criteria: Patients on insulin therapy, having a history of thyroid disorders, smoking, ischemic heart disease, hypertension, other medical disorders, and those who did not participate regularly for the period of 12 weeks were excluded from the study.
Intervention: The patients practiced exercises after completing routine activities in the morning and at least 4 hours after lunch in the evening. No change in dosage of drugs was done during 12 weeks period.
Group 1(control n = 30) who performed walking 45 minutes twice daily at a normal pace in a ground.
Group 2 (study group n = 20) who performed various relaxation exercises.
Sub group 2A (n = 10)who performed various yogic relaxation exercises[13-18]
Pranayama: These exercises consists of various respiratory inhalation- exhalation techniques- various pranayama practiced were
  1. Nadi Suddhi
  2. Anuloma-Viloma
  3. Bhramari
Asanas: These exercises involve fixed postures predominantly affecting abdominal musculature- various asanas practiced were (figure 1).
Figure 1: Ardha matsyendrasana (The half-twist posture).
Asanas practiced in standing position:
  1. Ardha Cakrasana (half-wheel posture)
  2. Pada Hastasana (Forward-bend posture)
Asanas practiced in sitting position:
  1. Sasankasana (Moon posture)
  2. Pacimatanasana (Posterior stretching)
  3. Ardha matsyendrasana (The half-twist posture)
Asanas practiced in lying position:
  1. Bhujangasana (Serpent posture)
  2. Salabhasana (Locust posture)
Kriya: These exercises involves active movement of abdominal wall muscles-
  1. Agnisar kriya
Vipassana: Sub group 2B (n = 10) who performed Vipassana relaxation technique 45 minutes twice daily for 12 weeks. Vipassana is also known as silent meditation retreat. It requires the novice to maintain strict silence. Main steps of Vipassana are [25,26]
  1. Not killing any species.
  2. Not lying, therefore you pledge to remain fully silent (most important step, silence preserves mental energy).
  3. No sexual misconduct (men and women are strictly separated).
  4. No intoxicants.
  5. In simple words it is a method that is based upon spending less from brain i.e. no active mental work viz- reading, writing, and speaking. No distraction- no Television, no mobile, no laptop etc. Though it is said to be meditation but it is a relaxation technique. Person is asked not to concentrate actively. It rejuvenates exhausted brain and reduces stress.
Outcome measurement Changes in glycemic parameters, lipid profile, EEG and SSR after 12 weeks.
Results
  Group 1 Subgroup 2A Subgroup 2B
Mean age (years) 47 46 46
Male % 52% 53% 51%
Female % 48% 47% 49%
Positive family history 35% 33% 32%
Good physical activity 84 % 91% 90%
Pulse rate/minute 84 ± 3 80 ± 3 82 ± 4
Blood pressure mm Hg 132 ± 4/78 ± 3 126 ± 6/76 ± 4 138 ± 4/78 ± 3
Respiration rate/min 18 ± 3 17 ± 2 16 ± 2
W:H ratio initial 0.843 ± 0.037 0.831 ± 0.037 0.844 ± 0.029
After 12 weeks 0.849 ± 0.040 0.835 ± 0.040 0.803 ± 0.012
Significance (P) value   0.324 0.001
Table 1: Vital parameters & Waist: Hip ratio.
Mental health life style factors Group 1 Sub group 2A Sub group 2B
a) Working mental hours      
>7 hours 85% 82% 91%
b) Mental rest      
Week end 14% 15% 17%
Month end 26% 24% 28%
No clear rest 60% 61% 55%
c)Sleep duration      
< 7 hours 48% 51% 50%
d)Presence of above factors      
>5 years 92% 88% 86%
e)PSS (perceived stress scale) >34/68 81% 92% 90 %
Table 2: Assessment of stress factors/mental health.
Groups Group 1 Sub group 2A Sub group 2B
Initial FBG mg% 164.77 ± 19.89 155.20 ± 20.78 166.40 ± 19.31
FBG after 12 weeks mg% 154.33 ± 19.41 152.2 ±19.81 88.40 ± 6.85
Statistical significance ---- P 0.76 P < 0.001
Initial PPBG mg% 252.17 ± 38.20 233.00 ± 21.1 255.50 ± 39.86
PPBG after 12 weeks mg% 237.60 ±37.80 223.6±24.4 165.40 ±15.63
Statistical significance ----- P 0.27 P < 0.001
Initial HbA1c % 11.65 ± 1.19 11.10 ± 0.58 10.6 ± 0.81
 HbA1c after 12 weeks % 11.56 ± 1.26 10.83 ± 0.60 6.36 ± 0.76
Statistical significance ------ P 0.02 P < 0.001
Table 3: Glycemic parameters.
Groups Group 1 Subgroup 2A Subgroup 2B
 Mean serum cholesterol in mg%      
Initial 217.60 ± 20.48 211.90 ± 15.90 217.40 ± 25.31
After 12 weeks 219.00 ± 21.63 207.30 ± 15.17 205.00 ± 25.04
Statistical significance P 0.123 P 0.096
Mean serum HDL mg%      
Initial 36.93 ± 6.44 34.50 ± 5.40 33.20 ± 4.31
After 12 weeks 37.20 ± 5.64 35.40 ± 3.66 44.90 ± 3.90
Statistical significance P 0.353 P < 0.001
Mean serum LDL mg%      
Initial 151.80 ± 17.06 146.80 ±16.17 157.60 ± 20.88
After 12 weeks 151.73 ± 16.14 145.40 ± 14.39 139.90 ± 19.62
Statistical significance P 0.278 P 0.065
Mean serum VLDL mg%      
Initial 28.60 ± 6.95 31.40 ± 7.60 26.60 ± 6.19
After 12 weeks 28.20 ± 8.31 27.90 ±7.95 21.60 ± 5.56
Statistical significance P 0.921 P 0.025
Mean serum Triglycerides mg%      
Initial 143.00 ± 34.75 157.00 ± 38.02 133.30 ± 30.93
After 12 weeks 137.67 ± 38.92 136.00 ± 38.77 108.00 ± 27.81
Statistical significance P 0.907 P 0.032
Table 4: Lipid profile.
Table 5: EEG (synchronization in subgroup 2 B after 12 weeks).
Table 6: SSR (Reduction in sympathetic discharge in subgroup 2 B after 12 weeks).
Statistical Analysis
Collected data were entered in computer based Microsoft Excel sheet. Statistical analysis was done by SPSS 20.0
Figure 2: Faded appearance of brain- chronic exhaustion/stress Normal appearance of brain after Vipassana.
Discussion
Worldwide, the incidence of type-2 diabetes is on the rise. The role of chronic mental stress/exhaustion in diabetes remains unclear. A few studies have found association of chronic mental stress/mental exhaustion in diabetes patients. Changes in the blood glucose levels associated with transient stress are also reported [3-12]. Still data is lacking in using stress relaxation methods in management of diabetes. Therefore, this prospective study was planned to observe effect of various stress relaxation methods on glycemic control, lipid profile, EEG and basal sympathetic discharge; and a possible correlation among all these.
The study was done in Mittal hospital and Keshvam hospital Ajmer, India. 50 confirmed cases of type-2 diabetes were taken for the study. The study period was from January 2016 to December 2017. The cases were randomly divided into two groups; group 1 (control n = 30) and group 2 (study n = 20). The group 2 was further subdivided into 2 subgroups 2A (n = 10) and subgroup 2B (n = 10). Throughout the 12 weeks period, the patients were closely observed for symptoms and any discomfort.
The intervention was done in the form of various stress relaxation methods to all the groups and subgroups. Group 1 performed walking 45 minutes twice daily for 12 weeks. Group 2 was divided into 2 subgroups, subgroup 2A performed Pranayama, Asanas and Kriyas exercises and subgroup 2B performed purely mental relaxation exercise like Vipassana. Subgroup 2 an exercises, Pranayama aims at conservation and control of bioenergy by respiration and nervous control. Asanas and Kriyas consist mainly of postural exercises help in to conserve energy. In subgroup (2B) Vipassana gives rest to mind, a person is not supposed not to concentrate instead asked to allow the thoughts to float. Group 1 was asked to walk at a normal pace. Each group performed respective exercises for 45 minutes twice daily for 12 weeks.
Initially all groups and subgroups had comparable vital parameters and W: H ratio there was no statistical significant difference. (Group 1v/s/2A, 1v/s2B p > 0.05) (Table 1) After 12 weeks in W: H ratio there was statistical significant reduction in W: H ratio in sub group 2 B. (Group 1v/s/2A p > 0.05, 1v/s 2B p < 0.05). (Table 1) Initially stress scaling and mental health life style factors were suggestive of chronic stress and chronic mental exhaustion in all groups and subgroups. (Table 2)                
In the present study all groups and subgroups had poor glycemic control and deranged lipid profile at the beginning. (Table 3, 4) Initial FBG/PPBG/ HbA1c there was no statistical significant difference among groups and subgroups (group 1v/s 2A, 1v/s 2B p > 0.05). (Table 3) After 12 weeks FBG/PPBG/ HbA1c there was statistical significant reduction in glycemic parameters in sub group 2 B. (group 1v/s 2A p>0.05, 1v/s 2B p < 0.001). (Table 3) Initial Tgl, LDL, HDL there was no statistical significant difference among groups and subgroups. (Group1v/s 2A, 1v/s 2B p > 0.05) (Table 4)
After 12 weeks there was statistical significant improvement in lipid profile parameters in sub group 2 B. There was reduction in Tgl and increase in HDL levels in sub group 2 B. (Group 1v/s 2A p > 0.05, 1v/s 2B p < 0.001) (Table 4)
Initial EEG was suggestive of low voltage fast Beta activity in eye closure state in all groups and subgroups. This was suggestive of desynchronized activity found in stress and mental exhaustion.
After 12 weeks EEG showed increase in amplitude/voltage and synchronization in sub group 2 B. (Table 5) Initial SSR was suggestive of spike activity (high basal discharge in all groups and subgroups)
After 12 weeks SSR showed reduction in spike voltage and a dome shaped response in sub group 2 B. (Table 6)
We summarize the findings
Initially all groups and subgroups were comparable, there was no statistical difference in W: H ratio, glycemic parameters (FBG, PPBG, HbA1c) lipid profile, EEG, SSR findings viz:
  1. Chronic stress
  2. Chronic mental exhaustion
  3. Desynchronized activity (fast Beta) in EEG
  4. Increased basal sympathetic discharge (spike in SSR)
  5. Deranged glycemic parameters
  6. Deranged lipid profile
After 12 weeks sub group 2 B who practiced Vipassana showed
  1. Synchronization (8-10 Hz alfa activity) and increase in amplitude/voltage in EEG
  2. Reduction in basal sympathetic discharge (dome in SSR)
  3. Reduction in W: H ratio
  4. Significant improvement in glycemic parameters
  5. Significant improvement in serum Tgl and HDL values
The findings clearly indicate that initially patients had chronic stress/exhaustion (suggested by PSS, questionnaire and EEG changes), increased basal sympathetic activity (suggested by SSR) and diabetes. After 12 weeks correction of these parameters in mental relaxation group (subgroup 2B) clearly indicates presence of central role in diabetes. Whereas no significant change occurred in sub group 2A i.e. those who performed abdominal/respiratory exercises.
How changes occurred- Possible mechanism
  1. Synchronization in EEG- Initial EEG had low voltage fast Beta activity in eye closure state was suggestive of desynchronized activity found in stress and mental exhaustion [3]. After Vipassana there was synchronized 8-10 Hz alfa activity in EEG in subgroup 2B. Vipassana (mental inactivity, observe silently what is happening) technique gives rest to mind/reduces stress, by not speaking, writing, reading, in a peaceful environment. By reducing mental activity improves physiologically depleted neuro- transmitters and reduces stress. Synchronization improves over all cortical control over lower centers, cortical-hypothalamic signals. [3, 27-30]
  2. Reduction in basal sympathetic discharge- Initially SSR was associated with spike response in all groups, along with normal resting pulse rate suggestive of high basal sympathetic activity. After 12 weeks in subgroup 2B there was reduction in basal sympathetic discharge. (Dome shaped response in SSR) This possibly results from improvement in signals to hypothalamus from various cortical areas (neo cortex, cingulate gyrus) [3,30,31]. Hypothalamus is the highest centre generating basal autonomic rhythm.
  3. Correction of W: H ratio – Reduction in basal sympathetic discharge reduces insulin resistance and therefore acquired obesity. [32]
  4. Improvement in glycemic parameters, lipid profile- (correction in insulin metabolism) Reduction in basal sympathetic discharge is responsible for reduction in insulin resistance and improvement in insulin release via inhibition of increased sympathetic discharge at alfa 1 and alfa 2 sympathetic receptors. Increased sympathetic activity is associated with insulin resistance in liver, muscles, tissues via stimulation of alfa 1 receptors and impaired insulin release via alfa 2 receptors in pancreas [3]. Reduction in basal sympathetic discharge normalizes activity of Na+/K+ (sodium/potassium) pump, shift of K+, insulin release, insulin sensitivity, and supports glucose entry into liver, muscle and tissues. [3, 33-35]
Conclusion
Chronic mental exhaustion (physiological depletion of neurotransmitters) and chronic stress are desynchronized state of brain, associated with increased basal sympathetic discharge. Increase in sympathetic discharge produces insulin resistance, impaired insulin release and diabetes. Vipassana/mental rest reduces expenditure of neurotransmitters, reduces the increased mental speed, reduces stress and helps in synchronization of brain. Therefore reduces basal sympathetic discharge and helps in control of diabetes. (Improvement in glycemic and lipid parameters) (figure 2)
Clinical implications
Prevention/treatment of diabetes Pandemic
The study gives 2 important messages:
  1. Clear central role in etio-pathogenesis of diabetes
  2. Role of mental rest/reduction in stress is helpful in controlling diabetes. Vipassana is one method to reduce stress and exhaustion. Other methods which are helpful in day to day life can also be used.
1) Prevent chronic mental exhaustion (burn out)/stress
  1. Avoid excess use of laptop, mobile, computers and television.
  2. Spend less; avoid unnecessary speaking/reading/writing, to prevent depletion of neuro- transmitters, if you have already excess mental work.
  3. All efforts must start from school days, adequate balance between mental/physical activities.
  4. Take adequate mental rest daily/at week end/at month end.
  5. Listening light music
  6. Avoid hurriedness in completing work.
2) Replenish neurotransmitters
  1. Good sound sleep for around 7 hours to prevent/control diabetes. [36]
  2. Practice mental relaxation/ Vipassana
  3. Spending some time in garden to produce calmness in mind.
Acknowledgement: Arvind Sharma (EEG/SSR technician), Mrinalini Apurva (computer assistance)
 Conflict of Interest: None
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Citation: Tarun Saxena., et al. “Effect of Various Stress Relaxation Exercises on Electroencephalography, Sympathetic Skin Response and Type-2 Diabetes Mellitus”. Archives of Endocrinology and Diabetes Care 2.1 (2018): 164-175.
Copyright: © 2018 Tarun Saxena., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.